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MacConkey agar is a selective and differential culture medium for bacteria designed to selectively isolate Gram-negative and enteric (normally found in the intestinal tract) bacilli and differentiate them based on lactose fermentation.〔(【引用サイトリンク】title=tmc.edu )〕 The crystal violet and bile salts inhibit the growth of gram-positive organisms which allows for the selection and isolation of gram-negative bacteria. Enteric bacteria that have the ability to ferment lactose can be detected using the carbohydrate lactose, and the pH indicator neutral red. ==Contents== It contains bile salts (to inhibit most Gram-positive bacteria), crystal violet dye (which also inhibits certain Gram-positive bacteria), neutral red dye (which turns pink if the microbes are fermenting lactose). Composition: 〔http://www.microbelibrary.org/index.php/component/resource/laboratory-test/2855-macconkey-agar-plates-protocols〕 * Peptone - 17 g * Proteose peptone - 3 g * Lactose - 10 g * Bile salts - 1.5 g * Sodium chloride - 5 g * Neutral red - 0.03 g * Crystal violet - 0.001 g * Agar - 13.5 g * Water - add to make 1 litre; adjust pH to 7.1 +/- 0.2 There are many variations of MacConkey agar depending on the need. If the spreading or swarming of Proteus species is NOT required, sodium chloride is omitted. Crystal violet at a concentration of 0.0001% (0.001 g per litre) is included when needing to check if Gram-positive bacteria are inhibited. MacConkey with sorbitol is used to isolate E. coli O157, an enteric pathogen. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「MacConkey agar」の詳細全文を読む スポンサード リンク
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